The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.)
| Autor: | Popović Sanja J., Kostadinović Ljiljana M., Brkljača Jovana S., Krulj Jelena A., Manojlović Maja S., Solarov-Bodroža Marija I. |
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| Jazyk: | English<br />Serbian |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Food and Feed Research, Vol 41, Iss 2, Pp 147-152 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2217-5369 2217-5660 |
| DOI: | 10.5937/FFR1402147P |
| Popis: | Aiming to achieve a precise method with easy application, this study presents the development and validation of a fast method for quantification of vitamin DL-α-tocopherol in quinoa seeds. The methodology was based on an extraction procedure using ultrasonic bath and determination by normal-phase HPLC with UV-VIS detector. Validation parameters showed adequate linearity, relative standard deviations between 0.5 and 0.8% (n=10), limits of detection and quantification were 3.0 ng/mL and 11.0 ng/mL, respectively. Moreover, testing the robustness of the method suggested that it was not changeable with time or condition. The results showed that this method is accurate and simple and thus applicable in laboratories for determination of DL-α-tocopherol in quinoa seeds. |
| Databáze: | Directory of Open Access Journals |
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