Autor: |
Kun Wang, Zi-Hao Xing, Qi-Wei Jiang, Yang Yang, Jia-Rong Huang, Meng-Ling Yuan, Meng-Ning Wei, Yao Li, Sheng-Te Wang, Kun Liu, Zhi Shi |
Jazyk: |
angličtina |
Rok vydání: |
2019 |
Předmět: |
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Zdroj: |
Frontiers in Oncology, Vol 9 (2019) |
Druh dokumentu: |
article |
ISSN: |
2234-943X |
DOI: |
10.3389/fonc.2019.00080 |
Popis: |
Urokinase plasminogen activator receptor (uPAR), a member of the lymphocyte antigen 6 protein superfamily, is overexpressed in different types of cancers and plays an important role in tumorigenesis and development. In this study, we successfully targeted uPAR by CRISPR/Cas9 system in two human cancer cell lines with two individual sgRNAs. Knockout of uPAR inhibited cell proliferation, migration and invasion. Furthermore, knockout of uPAR decreases resistance to 5-FU, cisplatin, docetaxel, and doxorubicin in these cells. Although there are several limitations in the application of CRISPR/Cas9 system for cancer patients, our study offers valuable evidences for the role of uPAR in cancer malignancy and drug resistance. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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