Low-dose phase retrieval of biological specimens using cryo-electron ptychography

Autor: Liqi Zhou, Jingdong Song, Judy S. Kim, Xudong Pei, Chen Huang, Mark Boyce, Luiza Mendonça, Daniel Clare, Alistair Siebert, Christopher S. Allen, Emanuela Liberti, David Stuart, Xiaoqing Pan, Peter D. Nellist, Peijun Zhang, Angus I. Kirkland, Peng Wang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-020-16391-6
Popis: Cryo-electron microscopy is widely employed in structural biology and uses phase contrast imaging. Here, the authors employ electron ptychography, a quantitative phase retrieval method for high-contrast, low-dose phase imaging of cryo-state rotavirus and immature HIV-1 virus-like particles, and show that electron ptychography is more efficient for phase recovery than conventional phase contrast imaging.
Databáze: Directory of Open Access Journals