| Autor: |
Maria Augusta de Carvalho Silvello, Giovana Alves Gasparotto, Rosana Goldbeck |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Cleaner Chemical Engineering, Vol 6, Iss , Pp 100111- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2772-7823 |
| DOI: |
10.1016/j.clce.2023.100111 |
| Popis: |
This study developed a multienzyme preparation for the hydrolysis of microalgal biomass. Possible synergism between enzymes was investigated using carbohydrate-rich Chlorella vulgaris biomass. Under optimum experimental conditions, α-amylase (Termamyl®) at 0.05 g−1 substrate and amyloglucosidase at 0.2 g−1 substrate achieved a maximum reducing sugars yield of 5.639 ± 0.32 g L−1 at a biomass loading of 10 g L−1. Saccharomyces cerevisiae JAYET cells modified with cellulase genes produced 8.015 g L−1 ethanol from unenriched whole microalgal hydrolysate. With the developed enzyme preparation, no pretreatment was necessary to convert the carbohydrate-rich fraction of microalgal biomass into third-generation bioethanol. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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