A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the 'Hook Effect'
| Autor: | Shuang Han, Yifeng Xue, Junlan Zhang, Jianrong Huang, Xiuxia Liu, Yankun Yang, Zhonghu Bai, Chunxin Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Journal of Analytical Methods in Chemistry, Vol 2020 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2090-8865 2090-8873 |
| DOI: | 10.1155/2020/8891437 |
| Popis: | A chemiluminescent immunoassay for human serum osteocalcin, or bone Gla protein, was established using a double-antibody sandwich model. Examination of the hook effect revealed that it was significantly reduced, and no hook effect was observed at an osteocalcin concentration of 4000 ng/mL. The established method showed good analytical performance and thermal stability. The limit of detection was 0.03 ng/mL. The intra-assay coefficient of variation (CV) was 3.22%–5.64%, the interassay CV was 4.42%–7.25%, and the recovery rate was 93.22%–107.99%. Cross-reactivity (CR) was not observed with bovine, rat, mouse, rabbit, or porcine samples. The developed method showed a good correlation with the N-MID product from Roche. In total, 1069 clinical patient samples were measured using the reagent kit developed in this study. |
| Databáze: | Directory of Open Access Journals |
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