Effects of Calcium Regulating Hormones on Osteoclast-Like Cell Formation in Hen Medullary Bone Marrow Culture
| Autor: | Toshie Sugiyama, Midori Sakurai, Shinji Hiyama, Seiji Kusuhara |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | The Journal of Poultry Science, Vol 41, Iss 4, Pp 307-314 (2004) |
| Druh dokumentu: | article |
| ISSN: | 1346-7395 1349-0486 |
| DOI: | 10.2141/jpsa.41.307 |
| Popis: | In order to examine the effects of the calcium regulating hormones on the differentiation of hen medullary bone osteoclasts, isolated medullary bone marrow cells were cultured for 8 days in media including parathyroid hormone (PTH), 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] or 17β-estradiol (E2), respectively. After 8 days of culture, tartrate-resistant acid phosphatase (TRAP) activity, a marker for osteoclasts, was enzymehistochemically detected on the cultured marrow cells and these cells were also stained with Mayer’s hematoxylin. Thereafter, TRAP-positive multinuclear cells were counted as osteoclast-like cells, and the effects of the calcium regulating hormones on the osteoclast differentiation from medullary bone marrow cells were indicated as the percentage (%) of the controls when vehicle was added in place of the hormones. As a result, treatment with PTH or 1,25(OH)2D3 significantly stimulated the differentiation of osteoclast-like cells, up to 218.3% or 159.2%, respectively. On the other hand, E2 treatment resulted in a moderate, but not significant, suppression of differentiation (85.8% of controls). These results indicate that PTH and 1,25(OH)2D3 stimulate medullary bone osteoclast differentiation, but E2 is likely inhibitory. |
| Databáze: | Directory of Open Access Journals |
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