| Autor: |
Mats T. Vervoorn, MD, Elisa M. Ballan, MSc, Sjoerd van Tuijl, MSc, Saskia C.A. de Jager, PhD, Selma E. Kaffka genaamd Dengler, MD, Joost P.G. Sluijter, PhD, Pieter A. Doevendans, MD, PhD, Niels P. van der Kaaij, MD, PhD |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
JHLT Open, Vol 3, Iss , Pp 100042- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2950-1334 |
| DOI: |
10.1016/j.jhlto.2023.100042 |
| Popis: |
Background: Ex situ heart perfusion is associated with a significant decline in graft quality related to oxidative stress, inflammation, endothelial dysfunction, and metabolic perturbations. We assessed the effects of a more optimized, cardioprotective normothermic perfusion approach compared to a conventional perfusion protocol in a slaughterhouse model using porcine hearts. Methods: A total of 12 hearts were harvested and subjected to 4 hours of normothermic perfusion. The optimized protocol consisted of an adenosine-lidocaine cardioplegic solution, subnormothermic initial reperfusion and controlled rewarming, hemofiltration and supplementation of methylprednisolone and pyruvate. This was compared to a conventional protocol consisting of St. Thomas II cardioplegic solution, normothermic initial reperfusion without hemofiltration or methylprednisolone, and a mixture of glucose and insulin for metabolic support. Results: Myocardial function was superior in the optimized group, while significant functional decline was absent. Hearts subjected to the conventional protocol demonstrated a significant reduction in function over time. Conclusions: We have developed a further optimized, cardioprotective normothermic ex situ heart perfusion approach and demonstrated significantly improved myocardial function and attenuated functional decline during 4 hours of normothermic perfusion, indicating improved preservation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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