Effects of triamcinolone acetonide on human trabecular meshwork cells in vitro

Autor: Ashish Sharma, A Jayaprakash Patil, Navin Gupta, M F Estrago-Franco, Saffar Mansoor, Vincent Raymond, M Cristina Kenney, Baruch D Kuppermann
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Mean deviation
non-proliferative diabetic retinopathy
short fluctuations
short wave automated perimetry
standard automated perimetry
Antimicrobial effect
conventional silicone oil
endophthalmitis agents
heavy silicone oil
Keratoconus
modified deep anterior lamellar keratoplasty
steep corneal curvature
Bevacizumab
branch retinal vein occlusion
intravitreal
macular edema
triamcinolone
Blindness
disability evaluation
quality of life
visual acuity
Mitomycin C
pterygium recurrence
pterygium surgery
subconjunctival bevacizumab injection
Imaging in glaucoma
optical coherence tomography
pediatric
retinal nerve fiber layer
Autogenous tissue grafting
lacrimal drainage system
lacrimal fossa
bypass surgery
Cornea
excimer laser
growth factors
myopia
photorefractive keratectomy
Triamcinolone acetonide
human trabecular meshwork cells
in vitro
Ophthalmology
RE1-994
Zdroj: Indian Journal of Ophthalmology, Vol 62, Iss 4, Pp 429-436 (2014)
Druh dokumentu: article
ISSN: 0301-4738
1998-3689
DOI: 10.4103/0301-4738.121143
Popis: Aim: To study the effects of triamcinolone acetonide (TA) on cultured human trabecular meshwork (HTM) cells. Materials and Methods: HTM cells were cultured and treated with 125, 250, 500 and 1000 μg/mL concentration of TA for 24 h. The cells were treated with both crystalline TA (TA-C) (commercial preparation) and solubilized TA (TA-S). Cell viability was measured by a trypan blue dye exclusion test. The activity of caspse-3/7 was measured by a fluorescence caspase kit and DNA laddering was evaluated by electrophoresis on 3% agarose gel. Levels of lactate dehydrogenase (LDH) were assessed with LDH cytotoxicity assay kit-II. Results: Mean cell viabilities of HTM cells after 24 h exposure to TA-C 125, 250, 500, and 1000 μg/mL were 75.4 ±2.45% (P < 0.0001), 49.43 ± 1.85% (P < 0.0001), 17.07 ± 2.39% (P < 0.0001), and 3.7 ± 0.9% (P < 0.0001), respectively, compared with the untreated HTM cells 92.49 ± 1.21%. The mean cell viabilities with 125, 250, 500, and 1000 μg/mL of TA-S were 94.47 ± 1.60% (P > 0.05), 90.13 ± 0.40% (P < 0.01), 85.57 ± 0.47% (P < 0.001), and 71.67 ± 3.30% (P < 0.0001), respectively, compared to DMSO-equivalent cultures. Untreated HTM control had a cell viability of 96.57 ± 1.98%. DMSO-treated controls of 125, 250, 500, and 1000 μg/mL had a cell viability of 94.73 ± 0.57%, 96.97 ± 1.08%, 93.97 ± 1.85%, and 97.27 ± 1.15%, respectively. There was no increase of caspase-3/7 activity in cultures treated with either TA-C or TA-S. DNA laddering showed no bands in the TA-C or TA-S treated cultures. There were significantly higher LDH release rates at all concentrations of TA-C compared to TA-S. Conclusions: Results show that the effect of TA-C and TA-S on HTM cells is due to cell death by necrosis at all concentrations except 125 μg/mL of TA-S. Elevated levels of LDH confirmed necrotic cell death. Our study also infers the relative safety of TA-S over TA-C.
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