LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark
| Autor: | Muhamad Salman Fareza, Nur Amalia Choironi, Sri Sutji Susilowati, Melina Puspita Rini, Vyola Festihawa, Irfansyah Septian Nur Fauzi, Esti Dyah Utami, Sarmoko Sarmoko |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Indonesian Journal of Pharmacy, Pp 356-364 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2338-9427 2338-9486 |
| DOI: | 10.22146/ijp.1328 |
| Popis: | Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC50 values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth. |
| Databáze: | Directory of Open Access Journals |
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