HPLC ANALYSIS OF PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF AMORPHA FRUTICOSA L. EXTRACTS
| Autor: | Bianca IVĂNESCU, Cristina LUNGU, Laurian VLASE, Adina Catinca GRĂDINARU, Cristina TUCHILUŞ |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Journal of Plant Development, Vol 26, Pp 77-84 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2065-3158 2066-9917 |
| DOI: | 10.33628/jpd.2019.26.1.77 |
| Popis: | The present study focuses on the chemical and biological analysis of the ethanolic 70% v/v extracts obtained from Amorpha fruticosa leaves, branches and fruits. The total phenolic content determined by Folin-Ciocalteu method was the highest in leaves extract (159.5 mg/g). The HPLC-MS analysis indicated the presence of ferulic acid, luteolin and rutoside in all three extracts. The antioxidant activity was tested by the DPPH method and the results indicated a good scavenging activity (EC50 = 18.8 ± 0.2 µg/mL) for the fruit extract, followed by leaf extract (EC50 = 38.03 ± 0.75 µg/mL) and branch extract (EC50 = 221.16 ± 1.7 µg/mL). The antimicrobial activity evaluated by agar disc diffusion method indicated the inhibition of growth for Staphylococcus aureus and Sarcina lutea for all samples. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|