Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box

Autor:	Mahdi Muhammad Moosa, Phoebe S. Tsoi, Kyoung-Jae Choi, Allan Chris M. Ferreon, Josephine C. Ferreon
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	transcription factors DNA-protein interactions Sox2 sequential DNA loading smFRET DNA conformational landscape sequential DNA bending transcription factor dosage Biology (General) QH301-705.5 Chemistry QD1-999
Zdroj:	International Journal of Molecular Sciences, Vol 19, Iss 12, p 3865 (2018)
Druh dokumentu:	article
ISSN:	1422-0067
DOI:	10.3390/ijms19123865
Popis:	Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluorescence spectroscopy, we show that Sox2 alone can modulate DNA structural landscape in a dosage-dependent manner. We propose that such stoichiometric tuning of regulatory DNAs is crucial to the diverse biological functions of Sox2, and represents a generic mechanism of conferring functional plasticity and multiplicity to transcription factors.
Databáze:	Directory of Open Access Journals
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