Multidimensional LC–MS/MS analysis of liver proteins in rat, mouse and human microsomal and S9 fractions

Autor: Makan Golizeh, Christina Schneider, Leanne B. Ohlund, Lekha Sleno
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: EuPA Open Proteomics, Vol 6, Iss C, Pp 16-27 (2015)
Druh dokumentu: article
ISSN: 2212-9685
DOI: 10.1016/j.euprot.2015.01.003
Popis: Liver plays a key role in metabolism and detoxification, therefore analysis of its proteome is relevant for toxicology and drug discovery studies. To optimize for high proteome coverage, protein and peptide-level ion exchange fractionation were assessed using rat liver microsomes and S9 fractions. 2D-(SCX-RP)-LC–MS/MS analysis with peptide fractionation was subsequently employed for rat, mouse and human samples, yielding between 1400 and 1939 identified proteins, 58% of which were shared between species, and with relatively high sequence coverage. This rich dataset is specifically interesting for the toxicology community, and could serve as an excellent source for targeted assay development.
Databáze: Directory of Open Access Journals