| Autor: |
Rick Laguna, Sarah J. Young, Chih-Chin Chen, Natividad Ruiz, Shang-Tian Yang, F. Robert Tabita |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Metabolic Engineering Communications, Vol 2, Iss , Pp 6-12 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2214-0301 |
| DOI: |
10.1016/j.meteno.2014.12.001 |
| Popis: |
Synthetic biology approaches for the synthesis of value-based products provide interesting and potentially fruitful possibilities for generating a wide variety of useful compounds and biofuels. However, industrial production is hampered by the costs associated with the need to supplement large microbial cultures with expensive but necessary co-inducer compounds and antibiotics that are required for up-regulating synthetic gene expression and maintaining plasmid-borne synthetic genes, respectively. To address these issues, a metabolism-based plasmid addiction system, which relies on lipopolysaccharide biosynthesis and maintenance of cellular redox balance for 1-butanol production; and utilizes an active constitutive promoter, was developed in Escherichia coli. Expression of the plasmid is absolutely required for cell viability and 1-butanol production. This system abrogates the need for expensive antibiotics and co-inducer molecules so that plasmid-borne synthetic genes may be expressed at high levels in a cost-effective manner. To illustrate these principles, high level and sustained production of 1-butanol by E. coli was demonstrated under different growth conditions and in semi-continuous batch cultures, in the absence of antibiotics and co-inducer molecules. Keywords: Bioproduct synthesis, Plasmid addicted, 1-Butanol, Constitutive promoter |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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