Rounding up the annual ryegrass genome: High-quality reference genome of Lolium rigidum

Autor: Jefferson Paril, Gunjan Pandey, Emma M. Barnett, Rahul V. Rane, Leon Court, Thomas Walsh, Alexandre Fournier-Level
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in Genetics, Vol 13 (2022)
Druh dokumentu: article
ISSN: 1664-8021
DOI: 10.3389/fgene.2022.1012694
Popis: The genome of the major agricultural weed species, annual ryegrass (Lolium rigidum) was assembled, annotated and analysed. Annual ryegrass is a major weed in grain cropping, and has the remarkable capacity to evolve resistance to herbicides with various modes of action. The chromosome-level assembly was achieved using short- and long-read sequencing in combination with Hi-C mapping. The assembly size is 2.44 Gb with N50 = 361.79 Mb across 1,764 scaffolds where the seven longest sequences correspond to the seven chromosomes. Genome completeness assessed through BUSCO returned a 99.8% score for complete (unique and duplicated) and fragmented genes using the Viridiplantae set. We found evidence for the expansion of herbicide resistance-related gene families including detoxification genes. The reference genome of L. rigidum is a critical asset for leveraging genetic information for the management of this highly problematic weed species.
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