The effect of statin administration on IL-6 and IL-1b expression in peripheral blood mononuclear cells of a hypertensive patient with SARS-CoV-2 spike protein stimulation
| Autor: | Andrianto Andrianto, Budi Susetyo Pikir, Rizki Amalia, Teuku Yusrizal, Fatimah Alzahra, Maya Qurota A’yun, Michael Jonatan |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Medicinski Glasnik, Vol 19, Iss 2, Pp 113-119 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1840-0132 1840-2445 |
| DOI: | 10.17392/1481-22 |
| Popis: | Aim The infection of the SARS-CoV-2 virus potentially causes a cytokine storm with elevated IL-6 and IL-1β levels. Statin therapy was common among COVID-19 patients due to their cardiovascular comorbidities. However, the effect of statins on COVID-19 infection is unclear. The aim of this study was to evaluate the impact of statin administration on IL-6 and IL-1β level in peripheral blood mononuclear cells (PBMCs) after SARS-CoV-2 spike protein stimulation. Methods The PBMCs were isolated from a hypertensive patient and stimulated by the SARS-CoV-2 subunit S1 spike protein. The PBMCs were then divided into four treatment groups and treated with simvastatin at various doses (10 µM, 25 µM, 50 µM, and control). IL-6 and IL-1β were measured from the supernatant using the ELISA method. Results The stimulation of SARS-CoV-2 spike protein in PBMC cell culture statistically increased IL-6 and IL1β expression of 5.2 and 35.07 fold, respectively (p |
| Databáze: | Directory of Open Access Journals |
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