| Autor: |
Lei Yan, Zhan Shihuai, Shi Xiaohua, Yang Lan, Wang Jiawei, Wang Shuiliang, Zhang Shenghang |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Xin yixue, Vol 54, Iss 6, Pp 397-402 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
0253-9802 |
| DOI: |
10.3969/j.issn.0253-9802.2023.06.005 |
| Popis: |
Objective To investigate the role of human umbilical cord mesenchymal stem cell-derived exosomes （hUC-MSC-Exo） in the tunicamycin-induced apoptosis of renal tubular epithelial cells （HKC）. Methods Endoplasmic reticulum stress （ERS） was induced by tunicamycin in HKC cells. Cell proliferation was detected by CCK-8 assay. The expression levels of ERS-related proteins of glucose-regulated protein 78 （GRP78），GRP94 and C/EBP homologous protein （CHOP） were determined by Western blot. Cell apoptosis was assessed by flow cytometry. hUC-MSC-Exo were isolated and identified by flow cytometry. The effects of different concentrations of hUC-MSC-Exo （0，40，60，80，100，150 and 200 μg/mL） upon cell activity were evaluated. Cell proliferation and apoptosis after co-treatment with hUC-MSC-Exo and tunicamycin were assessed by resazurin assay and flow cytometry. Results Treatment with 1，2 and 4 μg/mL tunicamycin could inhibit the proliferation of HKC in a concentration-dependent manner. Different concentrations of tunicamycin could induce ERS and cell apoptosis in HKC. Treatment with 100，150 and 200 μg/mL hUC-MSC-Exo could enhance cell proliferation. Compared with the 2 μg/mL tunicamycin group，cell proliferation was significantly increased in the 2 μg/mL tunicamycin and 150 μg/mL hUC-MSC-Exo co-treatment group （P < 0.05）. The apoptosis rate in the 2 μg/mL tunicamycin group was （14.16±1.58）%，and （8.18±0.58）% in the 2 μg/mL tunicamycin and 150 μg/mL hUC-MSC-Exo co-treatment group，and the difference was statistically significant （P |
| Databáze: |
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| Externí odkaz: |
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