Molecular Evidence of Clonal Vibrio parahaemolyticus Pandemic Strains

Autor: Nandini Roy Chowdhury, Soumen Chakraborty, Thandavarayan Ramamurthy, Mitsuaki Nishibuchi, Shinji Yamasaki, Yoshifumi Takeda, Gopinath Balakrish Nair
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Zdroj: Emerging Infectious Diseases, Vol 6, Iss 6, Pp 631-636 (2000)
Druh dokumentu: article
ISSN: 1080-6040
1080-6059
DOI: 10.3201/eid0606.000612
Popis: The upsurge in worldwide incidence of Vibrio parahaemolyticus infection in the last 5 years has been attributed to the recent appearance of three serotypes with pandemic potential: O3:K6, O4:K68, and O1:K untypeable (KUT). Thirty-five strains of these serotypes, isolated from different countries over 4 years, were characterized by ribotyping and pulsed-field gel electrophoresis to determine their origin. The ribotypes of the strains of these serotypes were indistinguishable, except for a Japanese tdh- negative O3:K6 strain and a U.S. clinical O3:K6 isolate, which had slightly different profiles. The migration patterns of the NotI-digest of the total DNA of the strains were similar, and only slight variations were observed between the serotypes. By contrast, the O3:K6 and O1:KUT strains isolated before 1995 and strains of other serotypes had markedly different profiles. The O4:K68 and O1:KUT strains most likely originated from the pandemic O3:K6 clone.
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