| Autor: |
Andriani Angelopoulou, Angelos Papaspyropoulos, Argyris Papantonis, Vassilis G. Gorgoulis |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 2, Pp 101257- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101257 |
| Popis: |
Summary: The in vitro recapitulation of chromosomal rearrangements is a necessary tool for understanding malignancy at the molecular level. Here, we describe the targeted induction of a large chromosomal inversion (>3.7 Mbp) through CRISPR-Cas9-mediated genome editing. As inversions occur at low frequency following Cas9 cleavage, we provide a detailed screening approach of FACS-sorted, single-cell-derived clonal human bronchial epithelial cell (HBEC) cultures. The protocol provided is tailored to HBECs; however, it can be readily applied to additional adherent cellular models.For complete details on the use and execution of this protocol, please refer to Zampetidis et al. (2021). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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