Autor: |
Andriani Angelopoulou, Angelos Papaspyropoulos, Argyris Papantonis, Vassilis G. Gorgoulis |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
STAR Protocols, Vol 3, Iss 2, Pp 101257- (2022) |
Druh dokumentu: |
article |
ISSN: |
2666-1667 |
DOI: |
10.1016/j.xpro.2022.101257 |
Popis: |
Summary: The in vitro recapitulation of chromosomal rearrangements is a necessary tool for understanding malignancy at the molecular level. Here, we describe the targeted induction of a large chromosomal inversion (>3.7 Mbp) through CRISPR-Cas9-mediated genome editing. As inversions occur at low frequency following Cas9 cleavage, we provide a detailed screening approach of FACS-sorted, single-cell-derived clonal human bronchial epithelial cell (HBEC) cultures. The protocol provided is tailored to HBECs; however, it can be readily applied to additional adherent cellular models.For complete details on the use and execution of this protocol, please refer to Zampetidis et al. (2021). |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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