| Autor: |
Marwan Anoud, Emmanuelle Delagoutte, Quentin Helleu, Alice Brion, Evelyne Duvernois-Berthet, Marie As, Xavier Marques, Khadija Lamribet, Catherine Senamaud-Beaufort, Laurent Jourdren, Annie Adrait, Sophie Heinrich, Geraldine Toutirais, Sahima Hamlaoui, Giacomo Gropplero, Ilaria Giovannini, Loic Ponger, Marc Geze, Corinne Blugeon, Yohann Couté, Roberto Guidetti, Lorena Rebecchi, Carine Giovannangeli, Anne De Cian, Jean-Paul Concordet |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
eLife, Vol 13 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2050-084X |
| DOI: |
10.7554/eLife.92621 |
| Popis: |
Tardigrades are microscopic animals renowned for their ability to withstand extreme conditions, including high doses of ionizing radiation (IR). To better understand their radio-resistance, we first characterized induction and repair of DNA double- and single-strand breaks after exposure to IR in the model species Hypsibius exemplaris. Importantly, we found that the rate of single-strand breaks induced was roughly equivalent to that in human cells, suggesting that DNA repair plays a predominant role in tardigrades’ radio-resistance. To identify novel tardigrade-specific genes involved, we next conducted a comparative transcriptomics analysis across three different species. In all three species, many DNA repair genes were among the most strongly overexpressed genes alongside a novel tardigrade-specific gene, which we named Tardigrade DNA damage Response 1 (TDR1). We found that TDR1 protein interacts with DNA and forms aggregates at high concentration suggesting it may condensate DNA and preserve chromosome organization until DNA repair is accomplished. Remarkably, when expressed in human cells, TDR1 improved resistance to Bleomycin, a radiomimetic drug. Based on these findings, we propose that TDR1 is a novel tardigrade-specific gene conferring resistance to IR. Our study sheds light on mechanisms of DNA repair helping cope with high levels of DNA damage inflicted by IR. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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