| Autor: |
Clara Liu Chung Ming, Dillan Pienaar, Sahar Ghorbanpour, Hao Chen, Lynne Margaret Roberts, Louise Cole, Kristine C. McGrath, Matthew P. Padula, Amanda Henry, Carmine Gentile, Lana McClements |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Biology of Sex Differences, Vol 15, Iss 1, Pp 1-15 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2042-6410 |
| DOI: |
10.1186/s13293-024-00672-6 |
| Popis: |
Abstract Background Hypertensive disorders of pregnancy (HDP) affect 2–8% of pregnancies and are associated postpartum with increased cardiovascular disease (CVD) risk, although mechanisms are poorly understood. Methods Human induced pluripotent stem cells (iPSC)-derived cardiomyocytes, cardiac fibroblasts and coronary artery endothelial cells were cocultured to form cardiac spheroids (CSs) in collagen type-1 hydrogels containing 10% patient plasma collected five years postpartum [n = 5 per group: normotensive control, gestational hypertension (GH) and preeclampsia (PE)]. Plasma-treated CSs were assessed for cell viability and contractile function and subjected to immunofluorescence staining and imaging. A quantitative proteomic analysis of plasma samples was conducted (controls n = 21; GH n = 5; PE n = 12). Results Contraction frequency (CF) was increased in PE-treated CSs (CF: 45.5 ± 3.4 contractions/minute, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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