Using Bland–Altman Analysis to Identify Appropriate Clonogenic Assay Colony Counting Techniques
| Autor: | Seth Buryska BS, Sanjana Arji BS, Beverly Wuertz BA, Frank Ondrey MD, PhD |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Technology in Cancer Research & Treatment, Vol 22 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1533-0338 15330338 |
| DOI: | 10.1177/15330338231214250 |
| Popis: | Objective Determine the interchangeability of various methods utilized for counting colonies in clonogenic assays. Methods Clonogenic assays of 2 head and neck cancer cell lines were counted through 4 different counting modalities: Manual counting pen, via microscope, 1 publicly available automated algorithm, and a semiautomated algorithm presented by the authors. Each method counted individual wells (N = 24). Pen and microscopic counts were performed by 2 observers. Parameters included both low-growth (150 colonies/well) cell lines. Correlational and Bland–Altman analyses were performed using SPSS software. Results Interobserver manual pen count correlation R 2 value in both growth conditions was 0.902; controlling for only low-growth conditions decreased R 2 to 0.660. Correlation of microscopic versus pen counts R 2 values for observers 1 and 2 were 0.955 and 0.775, respectively. Comparing techniques, Bland–Altman revealed potential bias with respect to the magnitude of measurement ( P |
| Databáze: | Directory of Open Access Journals |
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