Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids

Autor: Nadeem Asad, Emily Smith, Sudeep Shakya, Sutton Stegman, Lisa Timmons
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Methods and Protocols, Vol 6, Iss 2, p 25 (2023)
Druh dokumentu: article
ISSN: 2409-9279
DOI: 10.3390/mps6020025
Popis: We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness.
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