Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
| Autor: | Gabriel Martín Alvarez, Sofía Villanueva, Elizabeth Breininger, Marisa Geller, Claudio Ruhlmann, Gabriel Carlos Dalvit, Pablo Daniel Cetica, Masashige Kuwayama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Open Veterinary Journal, Vol 14, Iss 5, Pp 1191-1198 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2226-4485 2218-6050 |
| DOI: | 10.5455/OVJ.2024.v14.i5.14 |
| Popis: | Background: Intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors. Aim: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with PLCɀ activity of both species. Methods: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples. Results: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages, and ICSI + chemical activation presented the highest 2 PN, cleavage and blastocyst rates (P [Open Vet J 2024; 14(5.000): 1191-1198] |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|