Growth of the microalgae Tetraselmis tetrathele and nitrate depletion in culture medium Guillard f/2 and Conway - doi: 10.4025/actascibiolsci.v35i2.13971

Autor: Anderson Alan da Cruz Coelho, Mário Ubirajara Gonçalves Barros, João Henrique Cavalcante Bezerra, José William Alves da Silva, Ricardo Lafaiete Moreira, Wladimir Ronald Lobo Farias
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: Acta Scientiarum: Biological Sciences, Vol 35, Iss 2 (2012)
Druh dokumentu: article
ISSN: 1679-9283
1807-863X
DOI: 10.4025/actascibiolsci.v35i2.13971
Popis: This study aimed to evaluate the effect of nutrient depletion during the cultivation of the microalgae Tetraselmis tetrathele, and to verify the algal growth and productivity in different culture media (Guillard f/2 and Conway). The experiment was conducted in nine-liter containers, with working volume of eight liters, salinity 35 ± 2‰, temperature 28 ± 1°C, with constant light and aeration, around 200 μE cm-2 s-1 and 3 L air min.-1. Results showed that microalgae development caused nutrient depletion, since we observed an inversely proportional relationship between nitrate concentration and algal biomass during the cultivation. Regarding the culture media, a higher algal productivity was observed in the cultivation developed in Conway medium (p < 0.05), obtaining at the end of the cultivation, biomass of 3.38 ± 0.02 g L-1. In the Guillard medium, only 2.47 ± 0.05 g L-1 was obtained. The cultivation in Conway medium presented better results concerning microalgae growth, with a more pronounced exponential phase. This was due to the higher nitrate availability, which was reduced more rapidly in the culture medium Guillard f/2.
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