Laser Microdissection and Laser Pressure Catapulting for the Generation of Chromosome-Specific Paint Probes
| Autor: | L. Schermelleh, S. Thalhammer, W. Heckl, H. Pösl, T. Cremer, K. Schütze, M. Cremer |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 27, Iss 2, Pp 362-367 (1999) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/99272rr04 |
| Popis: | Chromosome-specific paint probes provide a powerful tool with wide applications in cytogenetic analysis. Here, we present a new approach using UV-laser microbeam microdissection in combination with laserpressure catapulting, which allows the fast isolation of single chromosomes for the generation of chromosome-specific paint probes. To demonstrate the feasibility of this approach, single chromosomes were collected and amplified with degenerate oligonucleotide-primed PCR, hapten-labeled and hybridized onto normal metaphase spreads. Fluorescence in situ hybridization signals revealed specific painting of the respective chromosomes. |
| Databáze: | Directory of Open Access Journals |
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