| Autor: |
Dini Kesuma, Galih S. Putra, Tegar A. Yuniarta, Farida Suhud, I G.A. Sumartha, Sawitri Boengas, Melanny I. Sulistyowaty, Tjie Kok |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Journal of Pharmacy & Pharmacognosy Research, Vol 11, Iss 5, Pp 902-925 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
0719-4250 |
| DOI: |
10.56499/jppres23.1657_11.5.902 |
| Popis: |
Context: The COVID-19 pandemic in 2020 resulted in widespread mortalities due to cytokine storms in the affected patients. Macrophage migration inhibitory factor (MIF) is one of the most interesting targets in developing anti-COVID-19 drugs. Some thiourea compounds have been identified as having potential as MIF inhibitors. Aims: To investigate MIF inhibitory activity of N-benzoyl-N’-phenylthiourea derivatives. Methods: The study consists of in-silico activity prediction of designed compounds using a molecular docking approach against MIF protein (PDB ID: 1LJT). Afterwards, the designed compounds were synthesized and tested in vitro using the tautomerase activity approach. Results: The molecular docking study showed that all designed compounds possess comparable docking scores to the native ligand of the protein. MIF Assay performed on compounds (1) and (2) indicated a decrease in tautomerase activity of the MIF target protein of only 10.1 and 6.2%, respectively, compared to the positive control. Conclusions: In silico results predicted better bioactivity against MIF protein, but the result does not translate to the in vitro assay, where two of the designed compounds possess only low inhibitory activity. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
