ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA

Autor: Alexandre Coelho Serquiz, Raphael Paschoal Serquiz, Thayane Kerbele Oliveira das Neves Peixoto, Elizeu Antunes dos Santos, Hugo Alexandre Oliveira Rocha
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2018
Předmět:
Zdroj: Revista Brasileira de Fruticultura, Vol 40, Iss 1 (2018)
Druh dokumentu: article
ISSN: 1806-9967
0100-2945
DOI: 10.1590/0100-29452018098
Popis: ABSTRACT The benefits of various foods, such as fruits and vegetables, have been the focus of several studies aimed at improving welfare, health, and reducing the incidence of diseases. Therefore, the present paper objectified to investigate the presence of molecules of biotechnological and pharmacological interest in peel, seed, and pulp of Annona x atemoya Mabb. Aqueous extracts of the fruit parts were obtained with different buffers and assessed as to their protein and phenolic compounds content. The three parts of the fruit presented different proportions of these compounds when subject to different extraction conditions, with the highest concentrations of proteins being found in the seed and phenolic compounds in the peel of the studied fruit. Bioactive proteins (protease inhibitors and lectins) were detected through inhibitory tests for trypsin and chymotrypsin and hemagglutinating activity tests with human erythrocytes. A variation of 400 to 9600 inhibition units for the trypsin in the analyzed extracts, whereas for chymotrypsin a variation of 200 to 2500 in the inhibition units for the three fruit parts, considering the different extraction conditions were identified. The extracts obtained from the seeds and the peel presented titers higher than 0.9 hemagglutination units, suggesting that the by-products from the processing of A. x atemoya are potential sources of bioactive molecules.
Databáze: Directory of Open Access Journals
Externí odkaz: