| Autor: |
Su-Su Lu, Shan-Shan Liu, Xiao-Jun Fan, Xiao-Xiang Sun, Jiang-Hua Bian, Ji-Bing Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Guoji Yanke Zazhi, Vol 17, Iss 9, Pp 1639-1642 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1672-5123 |
| DOI: |
10.3980/j.issn.1672-5123.2017.9.08 |
| Popis: |
AIM:To research the effects of lithium chloride on transforming growth factor beta(TGF-β)and connective tissue growth factor(CTGF)in cultured human Tenon capsule fibroblasts(HTFs)and explore its mechanism.METHODS: HTFs were cultured and identified by vimentin staining with immunofluorescence and the morphological characteristics. The experimental group was processed 48h with LiCl in concentration of 80mmol/L, the control group without LiCl. The mRNA expression of TGF-β and CTGF in two groups were analyzed with real-time fluorescent quantitative polymerase chain reaction(real time-qPCR)and the protein expression was detected with Western blot. RESULTS:The cultured HTFs expressed TGF-β and CTGF. The mRNA expression of TGF-β and CTGF significantly decreased compared with the control group(t=20.042, 14.995, P<0.05). the protein expression of TGF-β and CTGF also decreased significantly compared with the control group(t=46.058、12.452, PCONCLUSION: The cultured HTFs can express TGF-β and CTGF in mRNA and proteins' level. LiCl can reduce the expression of TGF-β and CTGF both in gene and proteins' level. LiCl has the potential to modulate wound healing for glaucoma filtration surgery. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
