Seroprevalence of Ovine Brucellosis by Modified Rose Bengal Test and ELISA in Southern Punjab, Pakistan

Autor: Zahid Iqbal, Huma Jamil*, Zafar Iqbal Qureshi, Muhammad Saqib, Laeeq Akbar Lodhi, Muhammad Salman Waqas and Muhammad Safdar
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Pakistan Veterinary Journal, Vol 33, Iss 4, Pp 455-457 (2013)
Druh dokumentu: article
ISSN: 0253-8318
2074-7764
Popis: Pertaining to reported high prevalence of brucellosis in Pakistan, district Layyah on account of having thick sheep population was selected for this study. A total of 384 sheep blood samples were collected randomly from different selected private herds in the district, and tested through Modified Rose Bengal (mRB) test and Indirect Enzyme Linked Immunosorbent Assay (iELISA) for the serological analysis against the Brucella antibodies. Positive samples from these two tests were further subjected to Competitive Enzyme Linked Immunosorbent Assay (cELISA). The individual based seroprevalence of brucellosis in sheep was found to be 7.0% by mRB. Herd based prevalence was 42.5%. The highest (P
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