Time Sequential Single-Cell Patterning with High Efficiency and High Density

Autor: Yang Liu, Dahai Ren, Xixin Ling, Weibin Liang, Jing Li, Zheng You, Yaxiaer Yalikun, Yo Tanaka
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Sensors, Vol 18, Iss 11, p 3672 (2018)
Druh dokumentu: article
ISSN: 1424-8220
18113672
DOI: 10.3390/s18113672
Popis: Single-cell capture plays an important role in single-cell manipulation and analysis. This paper presents a microfluidic device for deterministic single-cell trapping based on the hydrodynamic trapping mechanism. The device is composed of an S-shaped loop channel and thousands of aligned trap units. This arrayed structure enables each row of the device to be treated equally and independently, as it has row periodicity. A theoretical model was established and a simulation was conducted to optimize the key geometric parameters, and the performance was evaluated by conducting experiments on MCF-7 and Jurkat cells. The results showed improvements in single-cell trapping ability, including loading efficiency, capture speed, and the density of the patterned cells. The optimized device can achieve a capture efficiency of up to 100% and single-cell capture efficiency of up to 95%. This device offers 200 trap units in an area of 1 mm2, which enables 100 single cells to be observed simultaneously using a microscope with a 20× objective lens. One thousand cells can be trapped sequentially within 2 min; this is faster than the values obtained with previously reported devices. Furthermore, the cells can also be recovered by reversely infusing solutions. The structure can be easily extended to a large scale, and a patterned array with 32,000 trap sites was accomplished on a single chip. This device can be a powerful tool for high-throughput single-cell analysis, cell heterogeneity investigation, and drug screening.
Databáze: Directory of Open Access Journals
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