Utilização de resíduos agroindustriais para a produção de proteases pelo termofílico Bacillus sp em fermentação submersa: otimização do meio de cultura usando a técnica de planejamento experimental Protease production using agroindustrial residues by thermophilic Bacillus sp in submerged fermentation: optimization of the culture medium using an experimental design approach
| Autor: | Silvania Alves Ladeira, Marcela Vicente Vieira Andrade, Andréia Boechat Delatorre, Victor Haber Perez, Meire Lélis Leal Martins |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 33, Iss 2, Pp 324-328 (2010) |
| Druh dokumentu: | article |
| ISSN: | 0100-4042 1678-7064 41301315 |
| DOI: | 10.1590/S0100-40422010000200018 |
| Popis: | Thermophilic Bacillus sp. SMIA-2, produced protease when grown on apple pectic, whey protein and corn step liquor medium, whose concentration was varied from 3 to 10 gL-1, according to the central composite design 2³. The experiments were conducted in shaker, at 50 °C, 150 rpm and initial pH 6.5. The results revealed that the culture medium affected both, cell growth and enzyme production. After graphical and numerical optimization procedure, the enzyme production reached its maximum value at 30 h fermentation, reaching, approximately, 70 U protein mg-1, suggesting that this process was partially associated to the growth. |
| Databáze: | Directory of Open Access Journals |
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