Effect of turmeric extract and dimethyl sulfoxide on buffalo semen freezability and fertility
| Autor: | R. I. EL-SHESHTAWY |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Bulgarian Journal of Veterinary Medicine, Vol 25, Iss 4, Pp 578-585 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1311-1477 1313-3543 |
| DOI: | 10.15547/bjvm.2020-0124 |
| Popis: | The freeze-thaw process results in structural and functional damages caused by overaccumulation of reactive oxygen species (ROS). Addition of antioxidants to semen extenders is of a great importance to overcome this oxidative damage. The study objective was to evaluate the consequence of using Tris-citric acid fructose egg yolk (TCFY) extender supplemented with a combination of turmeric extract and dimethyl sulfoxide (DMSO) [TTD] on sperm freezability and fertility. From five tubes (each containing 5 mL TCFY), the first tube contained neither turmeric extract nor DMSO and was kept as control. The other four tubes contained 1.5% DMSO plus 100 µL/5 mL, 200 µL/5 mL, 300 µL/5 mL and 400 µL/5 mL turmeric extract. Semen samples were pooled and extended to reach an application of 60×106 sperm/mL (TT1D to TT4D, respectively). Diluted semen was exposed to the freezing protocol. The post cooling results revealed significant improvement in percent of alive sper-matozoa in TT1D, significant decrease in sperm abnormalities in all concentrations used, significant improvement in intact acrosome percentage in TT1D, TT2D and TT4D. The post thawing results ex-hibited significant improvement in sperm motility in TT1D, TT2D and TT4D; significant amelioration of sperm membrane integrity (HOST) in TT1D, TT2D, TT3D and TT4D. Acrosome integrity was main-tained in all concentrations as in the control. It was concluded that TT1D revealed the best semen quality in cooled semen; and TT1D, TT2D exhibited the superior post thawing semen quality. In addition, conception rate (CR) of the post-thawed semen was ameliorated in TT1D, TT2D, TT3D and TT4D with TT1D being the best one with this regard. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|