| Autor: |
Évelyn Silva de Melo Soares, Camila Celeste Brandão Ferreira Ítavo, Luís Carlos Vinhas Ítavo, Geraldo Tadeu Dos Santos, Carlos Eduardo Domingues Nazário, Israel Shekinah Souza Soares, Leandro Fontoura Cavalheiro |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
PLoS ONE, Vol 17, Iss 2, p e0263071 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0263071 |
| Popis: |
Studies comparing methodologies for fatty acids are very important, since they can influence the quality and final quantification of the lipid fraction. Objective-to compare different extraction methods for total lipids and to evaluate the effect of these methodologies on the quantitative composition of fatty acids in milk of lactating ewes raised in tropical pastures. The methodologies used were simple direct transesterification, using the HPLC grade organic solvent n-hexane, Bligh; Dyer (cold extraction, extracting mixture proportions 5, 10, and 15 mL in a ratio of 2:1:1 (v/v/v) of chloroform-methanol-water). The fatty acid methyl esters were separated by gas chromatography coupled with a flame ionization detector (GC-FID). The results show that there was no significant difference (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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