| Autor: |
Stojanović Marija, Šćepanović Ljiljana, Bosnić Olivera, Mitrović Dušan, Jozanov-Stankov Olga, Šćepanović Vuk, Šćepanović Radomir, Stojanović Teja, Ilić Slobodan, Djurić Dragan |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Acta Veterinaria, Vol 66, Iss 1, Pp 26-36 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1820-7448 |
| DOI: |
10.1515/acve-2016-0002 |
| Popis: |
Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hоur incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 μmol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1:10). Homogenates were centrifuged at 10000 for 10 min at 4° C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
