| Autor: |
Aaron Paul Russell, Lobna eGhobrial, Craig Robert Wright, Severine eLamon, Erin eBrown, Michihiro eKon, Matthew eSkelton, Rodney eSnow |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Frontiers in Physiology, Vol 5 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1664-042X |
| DOI: |
10.3389/fphys.2014.00314 |
| Popis: |
The present study aimed to investigate whether skeletal muscle from whole body creatine transporter (CrT; SLC6A8) knockout mice (CrT-/y) actually contained creatine (Cr) and if so, whether this Cr could result from an up regulation of muscle Cr biosynthesis. Gastrocnemius muscle from CrT-/y and wild type (CrT+/y) mice were analysed for ATP, Cr, Cr phosphate (CrP) and total Cr (TCr) content. Muscle protein and gene expression of the enzymes responsible for Cr biosynthesis L-arginine:glycine amidotransferase (AGAT) and guanidinoacetate methyltransferase (GAMT) were also determined as were the rates of in vitro Cr biosynthesis. CrT-/y mice muscle contained measurable (22.3 ± 4.3 mmol.kg-1 dry mass), but markedly reduced (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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