AVALIAÇÃO DA EFICÁCIA DE CRIOPROTETORES PERMEANTES E NÃO PERMEANTES NO DESCONGELAMENTO RÁPIDO E LENTO DO SÊMEN CANINO
| Autor: | Amanda Carla Acipreste, Eduardo Paulino Costa, Fabrício Albani Oliveira, Sanely Lourenço Costa, Talita Fernandes Silva, Daniel Cortes Beretta |
|---|---|
| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Ciência Animal Brasileira, Vol 15, Iss 1, Pp 107-114 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1518-2797 1809-6891 |
| DOI: | 10.5216/cab.v15i1.26124 |
| Popis: | The aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection during five-weeks period, were used. The base diluent medium used in this study was tris-citrate added with 3% of dimethylformamide + 3% glycerol (D1), 3% dimethylformamide and trehalose (D2) and 4% glycerol (D3). At defrosting, half of the semen samples from each diluent medium was defrosted by rapid method in water-bath at 75 °C for seven minutes, followed by a new immersion at 37 °C for 1 minute. The other half of the samples was defrosted by slow method, in water-bath at 37 °C for 1 minute. The semen was evaluated for sperm progressive motility and vigor, besides membrane integrity. For this, the semen samples were submitted to either hyposmotic and membrane integrity tests of the plasmatic membrane and acrosome (fluorescence). The results indicated that the use of glycerol as cryoprotector in TRIS diluter provides greater efficacy in cryopreserving spermatozoa of the canine species, when compared to dimethylformamide associated with trehalose or glycerol. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|