| Autor: |
Andresa Guimarães, Juliana Macedo Raimundo, Raisa Braul Rodrigues, Maristela Peckle Peixoto, Huarrisson Azevedo Santos, Marcos Rogério André, Rosangela Zacarias Machado, Cristiane Divan Baldani |
| Jazyk: |
English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Revista Brasileira de Parasitologia Veterinária, Vol 28, Iss 1, Pp 180-185 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1984-2961 |
| DOI: |
10.1590/s1984-296120180088 |
| Popis: |
Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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