| Autor: |
Marta Bjornson, Xingshun Song, Abhaya Dandekar, Annaliese Franz, Georgia Drakakaki, Katayoon Dehesh |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 5, Iss 13 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.1519 |
| Popis: |
Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These chemicals then act as tools for identification of regulatory components that could not otherwise be detected by forward genetic screens due to gene family redundancy or mutant lethality. This protocol describes a chemical genetic screen using Arabidopsis thaliana seedlings, which has led to recognition of novel players in the plant general stress response. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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