Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland

Autor: Izabela Wróbel-Pawelczyk, Patrycja Ronkiewicz, Monika Wanke-Rytt, Dominika Rykowska, Aneta Górska-Kot, Katarzyna Włodkowska, Agnieszka Topczewska-Cabanek, Teresa Jackowska, Joanna Chruszcz, Walentyna Marchut, Agnieszka Mastalerz-Migas, Krzysztof Korzeniewski, GIL Study Team, Anna Skoczyńska, Krzysztof Trzciński
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Scientific Reports, Vol 12, Iss 1, Pp 1-11 (2022)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-022-09488-z
Popis: Abstract We investigated pneumococcal carriage among unvaccinated children under five years of age at a time when the conjugate polysaccharide vaccine (PCV) was introduced in Poland into the national immunization program (NIP). Paired nasopharyngeal swab (NPS) and saliva samples collected between 2016 and 2020 from n = 394 children were tested with conventional culture and using qPCR. The carriage rate detected by culture was 25.4% (97 of 394), by qPCR 39.1% (155 of 394), and 40.1% (158 of 394) overall. The risk of carriage was significantly elevated among day care center attendees, and during autumn/winter months. Among isolates cultured, the most common serotypes were: 23A, 6B, 15BC, 10A, 11A. The coverage of PCV10 and PCV13 was 23.2% (23 of 99) and 26.3% (26 of 99), respectively. Application of qPCR lead to detection of 168 serotype carriage events, with serogroups 15, 6, 9 and serotype 23A most commonly detected. Although the highest number of carriers was identified by testing NPS with qPCR, saliva significantly contributed to the overall number of detected carriers. Co-carriage of multiple serotypes was detected in 25.3% (40 of 158) of carriers. The results of this study represent a baseline for the future surveillance of effects of pneumococcal vaccines in NIP in Poland.
Databáze: Directory of Open Access Journals
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