Clinical usefulness of anti-M-type phospholipase-A-receptor antibodies in patients with membranous nephropathy and the comparison of three quantification methods
| Autor: | Yasuhiro Katsumata, Yuko Okamoto, Takahito Moriyama, Rina Moriyama, Manabu Kawamoto, Masanori Hanaoka, Keiko Uchida, Kosaku Nitta, Masayoshi Harigai |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Immunological Medicine, Vol 43, Iss 1, Pp 47-56 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2578-5826 25785826 |
| DOI: | 10.1080/25785826.2019.1710079 |
| Popis: | Associations between anti-M-type phospholipase A2 receptor (PLA2R) antibodies and disease activity and prognosis have been suggested in primary membranous nephropathy (MN); however, more evidence is needed. We aimed to establish a clinically useful method to measure anti-PLA2R antibodies. We developed a western blot assay and a cell-based enzyme-linked immunosorbent assay (ELISA). Anti-PLA2R antibodies were evaluated retrospectively using these assays and the commercial solid-phase ELISA. Anti-PLA2R antibodies were detected in 12, 6, and 12 out of 23 Japanese patients with biopsy-proven primary MN using the western blot, the cell-based ELISA, and the solid-phase ELISA, respectively. The samples of the lupus MN patients tested negative. The levels of proteinuria correlated moderately with the titres of anti-PLA2R antibodies measured by the three methods (r = 0.39–0.47). Anti-PLA2R antibodies were significantly associated with physicians’ decisions on immunosuppressive treatment without prior knowledge of anti-PLA2R antibody positivity (p |
| Databáze: | Directory of Open Access Journals |
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