An ethanol extract of Senduduk fruit (Melastoma malabathricum L) inhibits the expression of vascular endothelial growth factor and tumour necrosis factor alpha in HeLa cells
| Autor: | Deri Edianto, Aznan Lelo, Syafruddin Ilyas, Marline Nainggolan |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Medicinski Glasnik, Vol 17, Iss 2, Pp 414-419 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1840-0132 1840-2445 71980318 |
| DOI: | 10.17392/1182-20 |
| Popis: | Aim Senduduk fruit (Melastoma malabathricum L) is native to Indomalaya and is believed to possess anticancer activity. This study investigated antiangiogenic and anti-inflammation effects of an ethanolic extract of Senduduk fruit (EESF) on HeLa cells. Methods Cytotoxicity was assayed in HeLa cell cultures exposed to a concentration series of 500–7.8 µg/mL of EESF. IC50 was determined with a methylthiazol-tetrazolium (MTT) cell viability assay. Antiangiogenic and anti-inflammation activity was evaluated by an immunocytochemical assay of vascular endothelial growth factor (VEGF) and tumour necrosis factor alpha (TNF-α) expression in HeLa cells cultured with 1× or 2× IC50 or as a control without EESF. Results IC50 of the EESF was 956 µg/mL. The intensity of VEGF staining indicated moderate expression in HeLa cells in response to IC50, weak expression in response to 2×IC50, and strong expression in the absence of the EESF. While the intensity of TNF-α staining indicated moderate expression in HeLa cells in response to IC50 and to the absence of EESF, and weak expression in response to 2× IC50. Conclusion Senduduk fruit extract inhibited VEGF and TNF-α expression in HeLa cells in a concentration-dependent manner. |
| Databáze: | Directory of Open Access Journals |
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