Autor: |
Maqsood Ahmed, Matthew J. S. Owens, Enrique M. Toledo, Ernest Arenas, Mark Bradley, Charles ffrench-Constant |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Frontiers in Cell and Developmental Biology, Vol 9 (2021) |
Druh dokumentu: |
article |
ISSN: |
2296-634X |
DOI: |
10.3389/fcell.2021.755406 |
Popis: |
The development of efficient cell culture strategies for the generation of dopaminergic neurons is an important goal for transplantation-based approaches to treat Parkinson’s disease. To identify extracellular matrix molecules that enhance differentiation and might be used in these cell cultures we have used micro-contact printed arrays on glass slides presenting 190 combinations of 19 extracellular matrix molecules selected on the basis of their expression during embryonic development of the ventral midbrain. Using long-term neuroepithelial stem cells (Lt-NES), this approach identified a number of matricellular proteins that enhanced differentiation, with the combination of Sparc, Sparc-like (Sparc-l1) and Nell2 increasing the number of tyrosine hydroxylase+ neurons derived from Lt-NES cells and, critically for further translation, human pluripotent stem cells. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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