Anti-inflammatory effects of 4′-demethylnobiletin, a major metabolite of nobiletin

Autor: Xian Wu, Mingyue Song, Kanyasiri Rakariyatham, Jinkai Zheng, Shanshan Guo, Zhonghai Tang, Shuangde Zhou, Hang Xiao
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Journal of Functional Foods, Vol 19, Iss , Pp 278-287 (2015)
Druh dokumentu: article
ISSN: 1756-4646
43633897
DOI: 10.1016/j.jff.2015.09.035
Popis: Nobiletin, a citrus flavonoid, has been associated with various beneficial biological activities. 4′-Demethylnobiletin (4DN) is a major metabolite of nobiletin, and its tissue level was found to be much higher than that of nobiletin after oral administration of nobiletin in mice. Anti-inflammatory effects of 4DN were studied in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. The results showed 4DN not only dose-dependently inhibited LPS-induced nitric oxide production, but also significantly reduced expression of pro-inflammatory mediators, namely PGE2, IL-1β and IL-6. 4DN potently suppressed the expression of iNOS and COX-2 at both protein and mRNA levels. 4DN also inhibited nuclear translocation of NF-κB and AP-1. Furthermore, we demonstrated that 4DN activated transcription factor Nrf2 and its dependent genes including HO-1 and NQO1 whose expression may contribute to anti-inflammatory effects. The results demonstrated anti-inflammatory effects of 4DN and provided a scientific basis for using nobiletin as a nutraceutical to inhibit inflammation-driven diseases.
Databáze: Directory of Open Access Journals
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