Analytical performance evaluation of a rapid immunochromatographic test for the diagnosis of bovine brucellosis based on a recombinant BP26 protein

Autor: P.G. Souza, P.A. Lima, P.M. Soares Filho, R.N. Etges, R.R. Nicolino, A.G. Viana, T.A. Paixão, R.T. Fujiwara, R.L. Santos
Jazyk: English<br />Portuguese
Rok vydání: 2024
Předmět:
Zdroj: Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 76, Iss 5 (2024)
Druh dokumentu: article
ISSN: 1678-4162
48788570
DOI: 10.1590/1678-4162-13158
Popis: ABSTRACT Brucellosis is an important bacterial disease of global distribution with zoonotic potencial. Serological tests used in Brazil for diagnosis of bovine brucellosis, including the Rose Bengal test (RBT), 2-mercaptoethanol (2-ME), fluorescent polarization (FPA), and complement fixation (FC), are based on the smooth lipopolysaccharide antigen (S-LPS) of Brucella abortus. The aim of this study was to evaluate a recombinant BP26 protein used as antigen in a rapid lateral flow immunochromatographic assay (rBP26-LFIA) for serological diagnosis of bovine brucellosis. Analytical performance of rBP26-LFIA was evaluated in positive and negative bovine serum samples previously characterized by RBT and 2-ME. Estimates of analytical sensitivity and specificity were 73.91% and 97.14%, respectively. Bovine sera reactive to Neospora, Trypanosoma vivax or Leptospira were used to assess specificity because these diseases are commonly associated with abortion in cattle. In addition to a possible cross-reaction induced by commercial vaccines against Leptospira in serological tests for bovine brucellosis using S-LPS as an antigen. In conclusion, rBP26-LFIA, with its current standardization, had good analytical performance. However, a future evaluation of diagnostic performance by rBP26-LFIA with samples from regions with known prevalence is necessary for its recommendation for use in the Brazilian program for the control and eradication of bovine brucellosis.
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