| Autor: |
Ludmila Branco Macedo, Zélia Inês Portela Lobato, Sílvia Ligório Fialho, Aline de Marco Viott, Roberto Maurício Carvalho Guedes, Armando Silva-Cunha |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Brazilian Archives of Biology and Technology, Vol 56, Iss 6, Pp 932-941 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
1678-4324 |
| Popis: |
This study investigated the adjuvant potential of W/O/W multiple emulsions and microemulsions, comparing them with traditional aluminum hydroxide and oil-in-water emulsion adjuvants against bluetongue vaccine (BTV). Local inflammatory reactions were assessed in rabbits by measuring the temperature of the animals and the skin thickness at the site of application. Antibodies titers were determined by serum-neutralization test. Histological analyses of lesions at the site of adjuvants application were done. Results showed that multiple emulsion and microemulsion maintained their stability even in the presence of complex components and presented adequate characteristics for subcutaneous administration. They were able to induce immune response against BTV, but it was smaller than the traditional adjuvants. Despite microemulsion adjuvant showed lower antibodies titre, it was easier to prepare more stable at 4°C and it was the only one that did not induce any local reaction. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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