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Objective To investigate the effect of different concentrations of bufotalin (BT) on the proliferation, migration, invasion, and epithelial-mesenchymal transition of human colorectal cancer HCT116 cells. Methods CCK-8 assay was used to measure cell viability after 24 and 48 h of BT treatment at different concentrations (0, 10, 20, 40, 80, 160, and 320 nmol/L), and the half-maximal inhibitory concentration (IC50) was calculated. The plate colony formation assay was used to verify the effect of BT treatment at the concentrations of 0, 12.5, and 25.0 nmol/L for 14 days on the colony formation ability of HCT116 cells (established as groups A, B, and C). The wound healing assay and the Transwell assay were used to verify the effect of BT treatment at the concentrations of 0, 25, and 50 nmol/L for 24 h on the migration and invasion abilities of HCT116 cells (established as groups A, C, and D). Western blot was used to measure the protein expression levels of E-cadherin and N-cadherin in HCT116 cells after 24 h of treatment in groups A, C and D. Results CCK-8 assay showed that after HCT116 cells were treated by BT for 24 or 48 h, the inhibitory effect of BT on the proliferation of HCT116 cells increased significantly with the increase in the concentration of BT (F=2 106.00,3 725.00,P |
