In utero intraocular AAV injection for early gene expression in the developing rodent retina

Autor: Masahiro Yasuda, Sivapratha Nagappan-Chettiar, Hisashi Umemori
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 3, Pp 100742- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100742
Popis: Summary: The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the expression of transgenes early in the developing retina are essential to study visual system development. Here, we describe a protocol to express genes-of-interest in the developing mouse retina via in utero intraocular adeno-associated virus injections.For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2021).
Databáze: Directory of Open Access Journals