Autor: |
Javier Negueruela, Valerie Vandenbempt, Stephanie Talamantes, Francisco Ribeiro-Costa, Mariana Nunes, André Dias, Mayank Bansal, Esteban N. Gurzov |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
STAR Protocols, Vol 5, Iss 3, Pp 103297- (2024) |
Druh dokumentu: |
article |
ISSN: |
2666-1667 |
DOI: |
10.1016/j.xpro.2024.103297 |
Popis: |
Summary: Gene editing of human pluripotent stem cells is a promising approach for developing targeted gene therapies for metabolic diseases. Here, we present a protocol for generating a CRISPR-Cas12a gene knockout of protein tyrosine phosphatases in human embryonic stem cells. We describe steps for differentiating the edited clones into pancreatic islet-like spheroids rich in β-like cells. We then detail procedures for implanting these spheroids under the murine kidney capsule for in vivo maturation. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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