| Autor: |
Svetlana H Hristova, Alexandar M Zhivkov |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Journal of Applied Hematology, Vol 14, Iss 4, Pp 263-267 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1658-5127 |
| DOI: |
10.4103/joah.joah_96_23 |
| Popis: |
BACKGROUND: Dimethyl sulphoxide (DMSO, a small strongly polarized amphiphilic molecule) is used as a cryoprotector due to its possibility to easily penetrate cell membranes and to form strong H-bonds with water molecules, preventing by that arising of aqueous crystals. AIMS: We have investigated the vitality of hematopoietic stem cells after incubation at +4°C, +20°C and +37°C for 30 min, 60 min and 24 h in cryoprotective solution with 5% DMSO, and also after deep-freezing at –80°C and subsequent storage for 24 hours at –20°C and +4°C. MATERIALS AND METHODS: The vitality was evaluated counting the number of alive (uncoloured) and dead stem cells (blue coloured) using a staining test with Trypan blue dye. RESULTS: The obtained results disclose that DMSO has a cytotoxic effect which depends on the temperature and the time of cultivation: the cytotoxicity is insignificant at +4°C even for 24 hours; at +37°C about 90% cells remain vital for 1 hour, however after 24 hours they completely die out. CONCLUSION: Comparing the non-frozen cells with the deep-frozen at –80°C and subsequently defrozen cells in identical conditions (24 hours storage at +4°C) reveals that the freezing process causes an additional delayed damaging effect, which leads to death of half of the stem cells.. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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