Investigation of the Role of Virulence Gene in Biofilm Formation of Escherichia coli Obtained from Clinical Specimens in Baghdad
| Autor: | R Kadhim Mohammed |
|---|---|
| Jazyk: | English<br />French |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Archives of Razi Institute, Vol 77, Iss 2, Pp 915-921 (2022) |
| Druh dokumentu: | article |
| ISSN: | 0365-3439 2008-9872 |
| DOI: | 10.22092/ari.2022.357205.1995 |
| Popis: | Several strains of Escherichia coli (E. coli) cause many diseases, including gastrointestinal illness, urinary tract infections, pericarditis, and septicemia. The present study aimed to evaluate the prevalence of the Universal Stress Protein (USP) virulence gene and the level of antibiotic resistance patterns associated with biofilm formation of E. coli in patients with infected burns, wounds, and urinary tract infections. Cases were selected from two hospitals of Al-Yarmouk Educational Hospitals and Baghdad Medical City, Baghdad, Iraq. The clinical specimens were classified as E. coli according to CLSI. The frequency of the USP gene was determined using the PCR technique. The rate of biofilm formation and antibiotic resistance were determined using microplate and agar diffusion methods, respectively. The recorded data on the distribution of E. coli isolates indicated that 33 (66%) of isolates were recovered from females and 17 (34%) of them were obtained from males (P=0.02). The results of the distribution of the isolates indicated that 16 (32%) and 18 (36%) isolates were recovered from 10-20 and 21-30 and 31-40 years old participants, respectively. The recorded data revealed that the highest rate of E. coli isolates was obtained from urine samples while the lowest one was recovered from burn samples (P |
| Databáze: | Directory of Open Access Journals |
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